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Duoning provides customized bioreactors with volume of >20 L for suspension cell culture, as well as supporting systems such as liquid mixing, inactivation and emulsification. In addition, we can provide customers with process design, pipeline construction, system integration, bioreactor design and manufacturing, control software development and other services.

System features

These systems can be used for suspension culture, microcarrier culture for monoclonal antibody, vaccine or protein-based therapeutics production, to achieve multiple use in one tank.

Suspension and microcarrier culture using propulsion impeller or lifting impeller, low shear force, short mixing time, good mixing effect

Deep microbubble direct air supply/bubble-free air supply device provide good oxygen transfer, meets the requirements of high-density cell culture, and reduces the damage to cells

Using jacket and tank dual temperature PID control, temperature control is stable and accurate

Sterilization-in-place sampling/sampling valve group to ensure safe, reliable and convenient operation

Air intake/exhaust filter sterilization can be carried out independently to meet the requirements of filter replacement during the culture process

CO₂/O₂/AIR flow rate adopts thermal mass flow controller, to realize the automatic feedback control of gas flow rate

Dissolved oxygen control can adopt AIR/O₂ or AIR/O₂/N₂ mode. The DO control adopts unique PID control logic for stable and accurate control

Deep sparger and overlay air supply adopt different control methods in sterilization process, to ensure thorough sterilization and no dead end

Deep sparger uses a combination of hardware and software, effectively eliminate culture fluid reflux

PH control adopts time judgment to avoid the simultaneous addition of CO₂ and base

Peristaltic pump control allows external mobile peristaltic pump, easy to replace

Pipeline and frame adopt open design, easy to use and maintain

Pipeline adopts 3A standard, comply with GMP and BPE requirements

Connection seal of the mixing impeller in the tank effectively avoids dead end and is easy to clean

Pipeline construction is carried out in cleanroom to ensure welding quality

Material inlet and tank bottom valves can be sterilized separately

All the pipelines and tee connections on the tank body meet 3D requirements

Dual mechanical seal, sterilization-in-place; or bottom magnetic stirring

The control system adopts PLC control

Servo motor, large torque, realizes stable and reliable work

Powerful software functions, such as parameter setting, display control, test parameter, curve analysis, data storage, and output printing

System adopts electronic signature, which meets the requirements of GMP audit

Technical parameters

Tank body	Total volume	40 L	75 L	150 L
	Working volume	30 L	50 L	120 L
	Diameter-height ratio	1:1.2	1:1.2	1:1.2
	Material	316L SS
	Polishing rate	Internal: 0.2-0.4 μm; external: 0.8μm
	Manufacturing standard	ASME, PED, GB D1/D2, ISO9001:2000
		Material 316L stainless steel, jacket 304 stainless steel Polishing accuracy of the surface in contact with the material Ra≤0.4 Design pressure: 0.3 MPa, working pressure: 0.25 MPa Connection configuration: safety glass window, side view window, feed valve group port, foam sensor port, pressure sensor port, in-place sampling port, air intake port, bottom valve, inoculation valve group port, base feed port, safety bursting disc
Stir	Type	Top/bottom stir, Dual mechanical or magnetic drive, PID control, clean air lubrication (mechanical seal)
	Display	Digital display, ±1 rpm
	Stir speed	25~250 rpm, ±1 rpm
	Impeller	Plow type impeller
Detection probe	pH	2~12, PID control, accuracy: ±0.01, correlate with base and CO₂
	DO	0～200%, PID control, accuracy: ±1%, correlate with O₂, Air
	Liquid level	SS probe, PID control
	Temperature	Pt100, PID Control, Digital display, ±0.1℃
Gas control	Flowrate measurement	Thermal mass flow controller (optional)
	Gas	Four-gas control system (N_2,O_2,CO_2,Air) , solenoid valve control
	Intake filter	0.2 μm, PTFE
	Exhaust filter	0.2 μm, PTFE
	Exhaust condenser	316L SS
	Pressure regulation	Automatic pressure regulating system, including pressure sensor, and regulating valve
Liquid feed	Two peristaltic pump	Variable speed pump, and flow is accumulated: regulate pump speed according to control requirement, thus control flow rate.
Temperature	Control method	PID, Automatic sterilization; The temperature is controlled by the external heater and the cooling water under the control of the control system
	Control accuracy	Display accuracy: 0.1℃, control accuracy: ±0.1℃
	Control range	5 ℃~65 ℃ above cooling water temperature
Control system		Industrial programmable logic controller
Optional configurations		Weighing, tank lid automatic lifting
		AC380V two phase five wire, 15Kw
Water, electricity, gas requirements		Cooling water pressure: 0.15 MPa
		Cooling water temperature: not higher than 30 ℃
		Input air pressure: 0.2 MPa (subject to drying and pre-filtration)
		Input steam pressure: 0.18 MPa (must be pre-filtered)

For more information about custom systems, please contact your local representatives or technical support team of Duoning.

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Stainless Steel Suspension Cell Culture Bioreactor